Species determination in Liparis (Orchidaceae) based on morphological and molecular data on the territory of “Muravyevsky park of sustainable use of natural resources”
УДК 575.222.582.594.6(470)
DOI:
https://doi.org/10.14258/pbssm.2019014Keywords:
Amur region, ITS, Liparis, Orchidaceae, phylogenyAbstract
The species of the genus Liparis were sampled on the territory of “Muravyevsky park of sustainable useof natural resources”, initial definition of plants was conducted in nature. Morphological characters are insufficient forthe species determination of the genus Liparis, so for to assess species identity of representatives of natural populationsfragments of plants were taken for molecular phylogenetic studies. The internal transcribed spacers (ITS1 and ITS2)of fragment of 18S–26S nuclear ribosomal DNA were selected as a molecular marker for molecular analyses. Therepresentatives of the research populations of the genus Liparis have separated in the two well-supported clades in themolecular phylogenetic tree. The ITS sequences of analyzed plants of each clade have specific replacement. Analysis ofmorphological characters and molecular data allowed us to conduct species identification of representatives of naturalpopulations of the genus Liparis.
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