Molecular phylogenetic study of rare weed-field species of the genus Avena L.
УДК 575.17:582.52
DOI:
https://doi.org/10.14258/pbssm.2021022Keywords:
ITS, NGS, oats, phylogeny, PoaceaeAbstract
A molecular phylogenetic study of weed-field species of the genus Avena L. using marker sequences ITS1–5.8S rRNA gene–ITS2 was undertaken. In addition, next-generation sequencing (NGS) was performed on the Illuminaplatform for the ITS1 sequence and the beginning of the gene 5.8S rRNA. Sanger sequencing results revealed the separateclade of microspecies with a good level of support and small level of difference between themselves. According to NGSsequencing data, the two most abundant subgenomes in terms of the number of sequences were identified. Among thecommon sequences of hexaploids, those associated with the C-genome were not found. The presence of unique ribotypeswas shown for A. persica and A. georgica.
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References
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Badaeva E. D., Shelukhina O. Yu., Goryunova S. V., Loskutov I. G., Pukhalsky V. A. Phylogenetic Relationships of Tetraploid AB-Genome Avena Species Evaluated by Means of Cytogenetic (C-Banding and FISH) and RAPD Analyses // Journal of Botany, 2010. – Vol. 2010. – Article ID 742307. – 13 pp. DOI:10.1155/2010/742307
Baum B. R. Oats: wild and cultivated. A monograph of the genus Avena L. (Poaceae). – Canada, 1977. – 463 pp.
Clement M., Posada D., Crandall K. A. TCS: a computer program to estimate gene genealogies // Molecular Ecology, 2000. – Vol. 9, № 10. – P. 1657–1660. DOI:10.1046/j.1365-294x.2000.01020
Doyle J. J., Doyle J. L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue // PhytochemicalBulletin, 1987. – Vol. 19. – P. 11–15.
Ladizinsky G. Studies in Oats Evolution. – Heidelberg e.a.: Springer, 2012. – 87 pp.
Loskutov I. G., Rines H. W. Avena L. In: Kole C. (ed) Wild crop relatives: genomic and breeding resources. –Heidelberg: Springer, 2011. – P. 109–184.
Múrias dos Santos A., Cabezas M. P., Tavares A. I., Xavier R., Branco M. TCS BU: A tool to extend TCS network layout and visualization // Bioinformatics, 2016. – Vol. 32, № 4. – P. 627–628. DOI:10.1093/bioinformatics/btv636
Ridgway K. P., Duck J. M., Young J. P. W. Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trnL (UAA) intron // BMC Ecology, 2003. – Vol. 3, (8е).
White T. J., Bruns T., Lee S., Taylor J. W. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics // PCR protocols: a guide to methods and application. / Innis M. A., Gelfand D. H., Sninsky J. J., White T. J.(Eds) – New York: Academic Press, Inc., 1990. – P. 315–322.
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2021-09-14
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Molecular phylogenetic study of rare weed-field species of the genus Avena L.: УДК 575.17:582.52. (2021). Проблемы ботаники Южной Сибири и Монголии, 20(1), 108-111. https://doi.org/10.14258/pbssm.2021022
