Molecular cytogenetic study of Eranthis (Ranunculaceae)
УДК 582.675.1:576.316
DOI:
https://doi.org/10.14258/pbssm.2021060Keywords:
Chromosomes, Eranthis Salisb., fluorescence in situ hybridization, Ranunculaceae Juss, ribosomal DNA, winter aconiteAbstract
45S and 5S ribosomal DNA were originally localized on chromosomes of five species of winter aconits,namely, Eranthis cilicica, E. hyemalis (section Eranthis), E. pinnatifida, E. stellata и E. tanhoensis (section Shibateranthis).Fluorescence in situ hybridization was performed with oligonucleotide DNA probes Oligo-pTa71-2 and Oligo-5S rDNAof wheat that are complementary to 45S and 5S ribosomal DNA. In addition, oligonucleotide DNA probe (Oligo-5.8SrDNA-Ran, 50 b) for localization of 45S rDNA was designed and tested. This probe is based on the 5.8S rDNA sequencesof some species of fam. Ranunculaceae taken from GenBank. A specific hybridization of the Oligo-5S rDNA and Oligo5.8S rDNA-Ran probes with the chromosomes of Eranthis was shown. The use of the Oligo-pTa71-2 probe did not localizeclusters of 45S rDNA on chromosomes of studied species.
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References
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Badaeva E. D., Surzhikov S. A., Agafonov A. V. Molecular-cytogenetic analysis of diploid wheatgrass Thinopyrum bessarabicum (Savul. and Rayss) A. Löve // Comparative Cytogenetics, 2019. – Vol. 13, is. 4. – P. 389 -402.
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Erst A., Sukhorukov A., Mitrenina E., Skaptsov M., Kostikova V., Krivenko D., Chernisheva O., Troshkina V., Kushunina M., Xiang K., Wang W. Integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) from North Asia // PhytoKeys, 2020. – Vol. 140. – P. 75–100.
Goryachkina O. V., Badaeva E. D., Muratova E. N., Zelenin A. V. Molecular cytogenetic analysis of Siberian Larix species by fluorescence in situ hybridization // Plant Syst Evol, 2013. – Vol. 299. – P. 471–479.
Luo X., Liu J. Fluorescence in situ hybridization (FISH) analysis of the locations of the oligonucleotides 5S rDNA, (AGGGTTT)3 , and (TTG)6 in three genera of Oleaceae and their phylogenetic framework // Genes, 2019. – Vol. 10, is. 5. – P. 375.
Luo X., He Zh. Distribution of FISH oligo-5S rDNA and oligo-(AGGGTTT)3 in Hibiscus mutabilis L. // Genome, 2021.
Mlinarec J., Šatović Z., Mihelj D., Malenica N., Besendorfer V. Cytogenetic and phylogenetic studies of diploid and polyploid members of tribe Anemoninae (Ranunculaceae) // Plant Biology, 2012. – Vol. 14. – P. 525 -536.
Park S. Y , Jeon M. J., Ma S. H., Wahlsteen E., Amundsen K., Kim J. H., Suh J. K., Chang J. S., Joung Y. H. Phylogeny and genetic variation in the genus Eranthis using nrITS and cpIS single nucleotide polymorphisms // Horticulture, Environment, & Biotechnology, 2019. – Vol. 60. – P. 239–252.
Ribeiro T., Loureiro J., Santos C., Morais-Cecílio L. Evolution of rDNA FISH patterns in the Fagaceae // Tree Genetics & Genomes, 2011). – Vol. 7. – P. 1113–1122.
Roa F., Guerra M. Distribution of 45S rDNA sites in chromosomes of plants: Structural and evolutionary implications // BMC Evolutionary Biology, 2012. – Vol. 12. – P. 225.
Rukšāns J., Zetterlund H. Eranthis iranica (Ranunculaceae) Rukšāns and Zetterlund – new species of winter aconite from Iran // International Rock Gardener, 2018. – Vol. 108. – P. 2–19.
Stefanoff B. Weitere materialien zur flora Bulgariens // Izvestiya na Botanicheskiya Institut, Bd, 1963. – Vol. 11. – P. 151–157.
Tang Z., Yang Z., Fu S. Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis // Journal of Applied Genetics, 2014. – Vol. 55. – P. 313–318.
Yu Z., Wang H., Xu Y., Li Y., Lang T., Yang Z., Li G. Characterization of chromosomal rearrangement in new wheat – Thinopyrum intermedium addition lines carrying Thinopyrum – specific grain hardness genes // Agronomy, 2019. – Vol. 9. – P. 18.
Yurkevich O. Y., Samatadze T. E., Selyutina I. Y., Romashkina S. I., Zoshchuk S. A., Amosova, A. V., Muravenko O. V. Molecular cytogenetics of Eurasian species of the genus Hedysarum L. (Fabaceae) // Plants, 2021. – V. 10, is. 1. – P. 89.
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2021-09-20
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Molecular cytogenetic study of Eranthis (Ranunculaceae): УДК 582.675.1:576.316. (2021). Проблемы ботаники Южной Сибири и Монголии, 20(1), 305-308. https://doi.org/10.14258/pbssm.2021060
