The problem of identification of invasive species of Heracleum on the territory of the Komi Republic
УДК 582.893.6(470.13)
DOI:
https://doi.org/10.14258/pbssm.2021136Keywords:
DNA barcoding, Heracleum mantegazzianum, Heracleum sosnowsky, nvasive species, Komi Republic, invasive speciesAbstract
According to literature, Heracleum mantegazzianum Somm. et Lev. and H. sosnowskyi Manden. (Apiaceae Lindl.) can be considered as ecological twins in a number of traits. There are still many uncertainties in the taxonomy of the genus Heracleum, and there are cases of interspecific hybridization. The process of identification was not always carried out and documented well during the introduction of plants of this genus in many regions of Europe. The authors collected and analyzed samples of Heracleum sosnowskyi plants with different degrees of leaf blade dissecting near Syktyvkar (Komi Republic) and Apatity (Murmansk region). Nucleotide sequences of genes rbcL and matK, the intergenic spacer trnH-psbA of chloroplast DNA and sequences of ITS and ETS of nuclear DNA were obtained for collected samples of the rbcL and matK gene sequences as well as the ETS sequences for H. sosnowskyi species were obtained for the first time. Comparative analysis of nucleotide sequences of plants of the genus Heracleum allowed us to determine potential DNA markers for molecular identification of H. sosnowskyi species. It was shown that for identification of H. sosnowskyi plants, the sequences of rbcL and matK chloroplast genes, as well as the ITS2 nuclear DNA sequence do not have sufficient polymorphism among closely related species and, therefore, cannot be used for identification of H. sosnowskyi plants. Analysis of trnH-psbA and ETS sequences showed that regardless of the degree of leaf blade dissection and geographic origin, all selected samples can be attributed to H. sosnowskyi. For a definitive answer to this question, more data for the plant H. man-tegazzianum should be included in the analysis.
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