ABOUT TRAINING IN THE DETERMINATION OF TOCOPHEROLS IN VEGETABLE RAW MATERIAL BY HPLC

UDC 543.544.5.068.7

Authors

  • Svetlana Nikolayevna Petrova Ivanovo State University of Chemical Technology
  • Irina Aleksandrovna Maksimova Ivanovo State University of Chemical Technology
  • Anastasiya Ruslanovna Yeshchenko Ivanovo State University of Chemical Technology
  • Yelena Mikhaylovna Mineyeva Ivanovo State University of Chemical Technology

DOI:

https://doi.org/10.14258/jcprm.2020037383

Keywords:

tocopherols, HPLC, alkaline hydrolysis of tocopherols, cranberries, oat flakes

Abstract

Tocopherols, which are important biologically active substances, play the role of antioxidants. Synthesized tocopherols are usually in esterified form; their sample preparation for quantification by high performance liquid chromatography includes high temperature alkaline hydrolysis. Sample preparation of oil-containing objects having natural tocopherols in an unesterified form is carried out by direct extraction in a non-polar solvent. In this work, we determined the content of tocopherols in natural materials (the method of analysis of viscous particles) using the alkaline hydrolysis method and the direct extraction method in a non-polar solvent. It was shown that the use of normal-phase HPLC with a prepared extraction sample in hexane and low-temperature exposure allows us to determine both the total content of tocopherols and its individual forms. This option is less time consuming, requires fewer reagents, which significantly affects the cost of the analysis. It is possible to determine only the total number of tocopherols. At the same time, overestimated results were obtained, which are probably associated with the destructive destruction of both tocopherols and tocotrienols present in plants of plant origin, and one large peak, including all products, is observed in the chromatograms.

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Author Biographies

  • Svetlana Nikolayevna Petrova, Ivanovo State University of Chemical Technology

    кандидат химических наук, доцент, доцент кафедры технологии пищевых продуктов и биотехнологии

  • Irina Aleksandrovna Maksimova, Ivanovo State University of Chemical Technology

    магистрант

  • Anastasiya Ruslanovna Yeshchenko, Ivanovo State University of Chemical Technology

    магистрант

  • Yelena Mikhaylovna Mineyeva, Ivanovo State University of Chemical Technology

    магистрант

References

1. Ovchinnikov Yu.A. Bioorganicheskaya khimiya. [Bioorganic chemistry]. Moscow, 1987, 668 p. (in Russ.).
2. Taghvaei M., Jafari S.M. Jr. Association of Food Scientists & Technologists, 2013, vol. 53, pp. 1272–1282. DOI: 10.1007/s13197-013-1080-1.
3. Poznyakovskiy V.M., Drozdova T.M., Vloshchinskiy P.Ye. Fiziologiya pitaniya. [Physiology of nutrition]. Moscow, 2018, 432 p. (in Russ.).
4. Sizova N.V., Andreyeva N.Yu. Khimiko-farmatsevticheskiy zhurnal, 2007, vol. 41, no. 6, pp. 49–52. DOI: 10.30906/0023-1134-2007-41-6-49-52. (in Russ.).
5. Trineyeva O.V., Safonova Ye.F., Slivkin A.I. Sorbtsionnyye i khromatograficheskiye protsessy, 2014, vol. 14, no. 1, pp. 144–149. (in Russ.).
6. Trineyeva O.V. Vestnik VGU, seriya: khimiya, biologiya, farmatsiya, 2013, no. 1, pp. 212–224. (in Russ.).
7. Gornas P., Solivenb A., Seglinaa D. European Journal of Lipid Science and Technology, 2015, vol. 116, pp. 773–777. DOI: 10.1002/ejlt.201400566.
8. Gornas P. Food Chemistry, 2014, vol. 172, pp. 129–134. DOI: 10.1016/j.foodchem.2014.09.051.
9. Pycia K., Kapusta I., Jaworska G., Jankowska A. European Food Research and Technology, 2018, vol. 245, pp. 607–616. DOI: 10.1007/s00217-018-3184-3.
10. Yang Y., Yang Y., Lu D., Yin S., Yang D., Chen Y., Li Y., Sun C. Journal of Separation Science, 2018, vol. 41, pp. 1829–1838. DOI: 10.1002/jssc.201701393.
11. Ishimaru M., Haraoka M., Hatate H., Tanaka R. Food Analytical Methods, 2017, vol. 10, pp. 92–99. DOI: 10.1007/s12161-016-0553-z.
12. Tarhan I., Kara H. Analytical Methods, 2019, vol. 11, pp. 4681–4692. DOI: 10.1039/C9AY01525F.
13. Barros L., Pereira E., Calhelha R. C., Duenas M., Carvalho A. M., Santos-Buelga C., Ferreira C.F.R.I. Journal of Functional Foods, 2013, vol. 5, pp. 1732–1740. DOI: 10.1016/j.jff.2013.07.019.
14. Luttseva A.I., Maslov L.G., Seredenko V.I. Khimiko-farmatsevticheskiy zhurnal, 2001, vol. 35, no. 10, pp. 41–45. (in Russ.).
15. Kozlov E.I., Solunina I.A., Lyubareva M. L., Nadtochiy M.A. Khimiko-farmatsevticheskiy zhurnal, 2003, vol. 37, no. 10, pp. 50–53. (in Russ.).
16. GOST EN 12822-2014. Produkty pishchevyye. Opredeleniye soderzhaniya vitamina E (a-, b-, g- i d-tokoferolov) metodom vysokoeffektivnoy zhidkostnoy khromatografii. [GOST EN 12822-2014. Food products. Determination of vit-amin E (α-, β-, γ- and δ-tocopherols) content by high performance liquid chromatography]. Moscow, 2015, 16 p. (in Russ.).
17. GOST R 54634-2011. Produkty pishchevyye funktsional'nyye. Metod opredeleniya vitamina E. [GOST R 54634-2011. Functional food products. Method for determination of vitamin E]. Moscow, 2013, 15 p. (in Russ.).
18. GOST R 54949-2012 (ISO 6867:2000). Korma dlya zhivotnykh. Opredeleniye soderzhaniya vitamina E metodom vysokoeffektivnoy zhidkostnoy khromatografii. [GOST R 54949-2012 (ISO 6867: 2000). Pet food. Determination of vitamin E content by high performance liquid chromatography]. Moscow, 2014, 16 p. (in Russ.).
19. Kishchenko V.A., Levchuk I.V., Yefimenko S.G. Maslichnyye kul'tury: Nauchno-tekhnicheskiy byulleten' VNII maslichnykh kul'tur, 2008, no. 1(38), pp. 35–37. (in Russ.).
20. Vorob'yeva O.A.Fundamental'nyye i prikladnyye issledovaniya: problemy i rezul'taty, 2016, no. 24, pp. 147–151. (in Russ.).
21. Vorob'yeva O.A., Mel'nikova N.B. Mezhdunarodnyy zhurnal prikladnykh i fundamental'nykh issledovaniy, 2015, no. 10-2, pp. 295–303. (in Russ.).
22. Rudnev S.D., Grachev A.V., Lavrinova Ye.A. Nauchnyy zhurnal NIU ITMO. Seriya «Protsessy i apparaty pishchevykh proizvodstv», 2011, no. 2, pp. 186–192. (in Russ.).
23. Skurikhin I.M., Tutel'yan V.A. Khimicheskiy sostav rossiyskikh pishchevykh produktov. [The chemical composition of Russian food products]. Moscow, 2002, 237 p. (in Russ.).

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Published

2020-10-22

Issue

No. 3 (2020)

Section

Low-molecular weight compounds

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How to Cite

[1]
2020. ABOUT TRAINING IN THE DETERMINATION OF TOCOPHEROLS IN VEGETABLE RAW MATERIAL BY HPLC: UDC 543.544.5.068.7. chemistry of plant raw material. 3 (Oct. 2020), 225–231. DOI:https://doi.org/10.14258/jcprm.2020037383.

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